超高效液相色谱-串联质谱法测定猪肉中巴喹普林残留

determination of Baquiloprim residues in Pig muscle by UPLC-MS/MS

关键词:  超高效液相色谱-串联质谱  巴喹普林  残留  猪肉

Key words:  UPLC-MS/MS  Baquiloprim  residues  Pig muscle

摘要:

建立了猪肉中巴喹普林药物残留的超高效液相色谱-串联质谱测定方法,样品用乙酸乙酯提取,吹干后用60%乙腈水(0.1 %甲酸)溶解,供液相色谱-串联质谱法分析,内标法定量。结果表明：巴喹普林标准溶液在100～800 mg/L范围内呈现良好线性,r2均大于0.998；方法检出限为5 μg/kg,定量限为10 μg/kg；巴喹普林在20～80 μg/kg添加范围内的平均回收率为96.7%～108%,批内、批间RSD均小于3.9.%。该方法简便快捷、灵敏度高、定性准确,能满足食品安全检测有关法规的要求。

Abstract:

SA ultral performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for the determination of Baquiloprim residues in Pig muscle. The samples were extracted by Ethyl acetate ,After evaporation,Was dissolved by 60% acetonitril(0.1 %Formic acid)and was determinatied on UPLC-MS/MS. The method was quantified by Internalstandard method. The calibration curves were linear between the peak areas and the concentrations of 20～800 mg/L with correlation coefficients(r2)more than 0.998. The limit of detection of Baquiloprim was 5 μg/kg, and the limit of quantitation was 10 μg/kg. The average recoveries from spiked muscle at concentrations of 20～80 μg/kg ranged 96.7%～108% and the relative standard deviations of intra- and inter-batch were both less than 3.9%.