水貂 β-干扰素基因的克隆、测序与遗传进化分析

Cloning, Sequence and Genetic Evolution Analysis of Mink β- interferon Gene

关键词:  水貂  IFN-β基因  克隆测序  遗传进化

Key words:  mink  β-interferon gene  cloning and sequencing  heredity and evolution

摘要:

根据GenBank发表的序列,应用DNAstart分析软件设计并合成一对引物用于扩增水貂β干扰素(IFN-β)基因，从病死水貂的脾脏中提取总RNA，RT-PCR扩增水貂β干扰素基因,获得了约561 bp片段，将其克隆到pEasy-T1载体中，进行序列分析，证实该基因是水貂β干扰素基因。将测序结果与GenBank发表的雪貂（EF581890.1）的IFN-β基因序列进行比较，核苷酸序列同源性为100.0%，氨基酸同源性为100.0%。同时将核苷酸序列、氨基酸序列与其他几种犬科动物进行遗传进化分析，其同源性分别在 83.0%-100.0%之间和 69.4%-100.0%之间。

Abstract:

According to published GenBank sequences, a primer for the increase of mink beta- interferon (IFN- beta) gene was designed and synthesized by DNAstart analysis software, total RNA was extracted from the infected mink spleen, mink beta- interferon gene was increased about 561 bp through RT-PCR method. After this gene was cloned to pEasy-T1 carrier, the facts prove that this gene is mink beta- interferon according sequence analysis. The result suggests that beta-interferon nucleotide sequence homology are 100.0% and amino acid homology are also 100.0% when compared sequence result with published gene sequence(EF581890.1) from GenBank. The nucleotide sequence homology and amino acid homology are 83.0%-100.0% and 69.4%-100.0% respectively when compared with another canine animals.