改进试管凝集试验法检测布鲁氏菌病抗体

Detection of anti-Brucella abortus sera agglutination titers with modified tube agglutination

关键词:  布鲁氏菌病  试管凝集试验  微量试管凝集试验

Key words:  Brucella abortus  SAT  MSAT

摘要:

为证实改进的试管凝集试验（SAT）的使用价值，分别用微量试管凝集试验（MSAT）和SAT以国际标准血清为参比检测6份牛布鲁氏菌病阳性血清的抗体效价。结果表明：MSAT和SAT试验结果一致，而且MSAT具有稀释方法更简便、精确，省时省力，可节约血清、抗原等试验原材料，试验结果容易判定，重现性良好等优越性。

Abstract:

To prove the use value of the modified tube agglutination, six anti-Brucella abortus sera were detected with MSAT and SAT referred to the international standard. The results showed that the sera titers were almost consistent detected with SAT and MSAT and the advantages of MSAT could be list as follows: The dilution method was simpler and more accurate. We could save time and effort, and save the materials such as serum, antigen etc. The result was easier to determine and with good reproducibility. So we proposed that revising the standard in this experiment, SAT was replaced by MSAT.