鹅细小病毒荧光定量PCR检测方法的建立及应用

Establishment and Application of Fluorescence Quantitative PCR for Identification of Goose Parvovirus

关键词:  鹅细小病毒  荧光定量PCR  定植规律

Key words:  Goose parvovirus  the fluorescent quantitative PCR  the Laws of Colonization

摘要:

本研究利用已经分离得到的小鹅瘟病毒GPV SP株，设计合成1对特异性的引物和Taq Man 探针，通过特异性试验，敏感性试验建立了GPV Taq Man荧光定量PCR检测方法。同时饲养产蛋种鹅，接种小鹅瘟病毒，在不同的时间段采取病料，并且利用荧光定量PCR检测病毒的存在与含量，得到了小鹅瘟病毒在产蛋种鹅体内的定植规律。

Abstract:

In this study, the GPV SP strain was used to find out the selfish gene sequence of the GPV VP3 according to the Genbank. Then the fluorescent quantitative PCR method was developed for the detection of GPV based on the specific primer and the probe of Taq Man. The reaction system and reaction condition are optimized, and the standard curve was established to evaluate the specificity and sensitivity. In order to study the field planting disciplinarian of the GPV in the egg kind of goose, we inoculate the GPV virus to the goose, take the disease material at the different times, and then use the fluorescent quantitation PCR to detect the existence and content of the virus.