氟苯尼考混悬型微乳剂的急性毒性试验

Study on Acute Toxicity of Florfenicol Suspended Microemulsion to Mice

关键词:  氟苯尼考混悬型微乳剂  乳剂  急性毒性试验

Key words:  florfenicol suspension type microemulsion  emulsion  toxicity

摘要:

为评价氟苯尼考混悬型微乳剂的安全性，按改良寇氏法进行了氟苯尼考混悬型微乳剂的急性毒性试验。根据预实验结果，60只小鼠平均分为6组，雌雄各半，分别按6000、4558.5、3464、2632、2000 mg/kg氟苯尼考混悬型微乳剂和0.5 mL/kg生理盐水注射液给小鼠腹腔一次性注射，同时用氟苯尼考普通注射液做对照，重复上述试验，均连续观察7 d。结果显示：氟苯尼考混悬型微乳剂最大耐受量为6000 mg/kg，其LD50为3658.8 mg/kg，LD50 95％可信限为2075.8～5241.8 mg/kg。氟苯尼考注射液的LD50为636.68 mg/kg，LD50 95％可信限为538.5～735.1 mg/kg。此剂量氟苯尼考混悬型微乳剂中氟苯尼考的含量相当于临床推荐用量（20 mg/kg）的180倍，表明氟苯尼考混悬型微乳剂毒性显著降低，在治疗剂量范围内使用安全。

Abstract:

In order to evaluate the actue toxicity of f1orfenicol suspended microemulsion，modified Korbor’s method was used to study the acute toxicity test of florfenicol suspended microemulsion．On the basis of results from preliminary experiment，60 mice were randomly divided into 6 groups , which evenly divided between male and female．Among 5 groups of mice were respectively administered with 6000，4558.5，3464，2632，2000 mg/kg florfenicol suspended microemulsion and the rest group of mice were given 0.5 mL/kg normal sodium by intraperitoneal injection, and observed for 7 days continuously．The results indicated that the maximal tolerance dose of florfenicol suspended microemulsion was 6000 mg/kg， the LD50 was 3658.8mg/kg (which is 180 times of clinical dosage of 20 mg/kg)，and 95% confidence limit was 2075.8～5241.8 mg/kg．And the maximal tolerance dose of general florfenicol injection was 1000 mg/kg, the LD50 was 636.68 mg/kg, and 95% confidence limit was 538.5～735.1 mg/kg. In conclusion, the toxicity of florfenicol suspended microemulsion to mice was significantly lower than that of florfenicol．It was belonged to hypotoxicity and safe to clinical application.