| 摘要: |
| 用纯化的猪伪狂犬病病毒gB重组蛋白为抗原，建立了检测猪伪狂犬病血清抗体的gB-ELISA方法。最佳反应条件为：抗原包被浓度为3.15μg/ml，待检血清稀释度为1:40。该方法对猪圆环病毒病、猪瘟、猪细小病毒病、猪繁殖与呼吸综合征(猪蓝耳病)、猪乙型脑炎、猪布氏杆菌病5种疾病阳性血清和SPF猪阴性血清检测呈阴性反应。批间、批内试验变异系数均不超过8％。用该方法与HerdChek ELISA试剂盒同时对119份血清进行了平行检测，其相对敏感性、特异性和符合率分别为：75%、80.7%和79%。试验结果表明：猪伪狂犬病血清抗体gB-ELISA检测方法具有较高的敏感性和特异性，且重复性好，可用于猪伪狂犬病毒血清抗体检测。 |
| 关键词: 猪伪狂犬病毒 抗体 gB-ELISA |
| DOI： |
| 投稿时间：2011-04-25修订日期：2011-05-31 |
| 基金项目:“十一五”国家科技支撑项目(2006BAD6A13) |
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| Establishment of the Indirect ELISA for the Detection of Viral Glycoprotein B Antibodies against Swine Pseudorabies |
| luofei |
| (China Animal Health and Epidemiology Center) |
| Abstract: |
| The gB-ELISA was established by using the purified PRV gB recombinant protein as antigen for detecting PRV antibodies in pig sera. All reaction conditions of this gB-ELISA were explored. The optimal coating concentration of antigen is 3.15μg/ml，the serum sample was diluted to 1:40. The results are negative when the antiserum against PCV, PPV, HCV, JEV and PRRS were detected by this gB-ELISA．The CV % between and within batches of detection were less than 8％．119 pig sera samples were simultaneously detected by this indirect gB-ELISA and HerdChek gB ELISA kit．The relative sensitivity and specificity of indirect gB-ELISA established in the study were75% and 80.7%. The coincidence rate between these two ELISA was79%．All results showed that this assay had a good sensitivity，specificity and repeatability，it could be used for detection of PRV antibody in pig sera． |
| Key words: swine pseudorabies virus antibody gB-ELISA |
