复方黄芪金银花颗粒剂的质量标准研究

Study the standard of Huangjin Compound granules

关键词:  复方黄芪金银花颗粒剂  薄层色谱法  紫外分光光度法  绿原酸  

Key words:  Huangjin compound granules ,TLC, Dual-beam UV-visible spectrophotometry, Chlorogenic acid

摘要:

摘要：目的：建立控制复方黄芪金银花颗粒剂的质量标准。方法：采用薄层层析对颗粒中女贞子、金银花、黄芪、枸杞子进行了薄层鉴别；采用双光束紫外-可见光分光度法测定了颗粒中绿原酸的含量。结果：薄层鉴别方法专属性强，含量测定绿原酸在42.24—84.48ug /ml内具有良好的线性关系,标准曲线回归方程为：A=0.0053證－0.0012（r=0.9999，n=5）；样品在24h内稳定，RSD=0.733%；加样平均回收率为102.049%，RSD=0.499%；该方法精密度高，RSD=0.382%。结论：本质量标准可有效地控制复方黄金颗粒剂的质量。

Abstract:

Abstract: Objective: Establish control standards of Huangjin compound granules.Methods: Using the Thin layer chromatography to distinguish Astragalus, Lonicera japonica, Ligustrum lucidum, Chinese wolfberry fruit, etc. in the particles. Dual-beam UV - visible spectrophotometry in the determination of the particle content of chlorogenic acid. Results: TLC method is specific. Determination of chlorogenic acid has good linearity within the the 42.24-84.48ug / ml .The Standard curve regression equation: A=0.0053證－0.0008（r=0.9999，n=5）. The Sample is stable in the 24h, RSD=0.733%. The average recovery is 102.049%, RSD=0.499%. This method was high Precision, RSD=0.382%.Conclusion: The quality standards that can effectively control the quality of Huangjin compound granules.