猪K88、K99、987P、F41埃希氏大肠杆菌高密度发酵培养技术的初步探讨

STUDY ON HIGH DENSITY FERMENTATION OF ESCHERICHIA COLI K88，K99，987P AND F41

关键词:  猪埃希氏大肠杆菌  K88、K99、987P、F41  菌毛抗原  高密度发酵培养

Key words:  Escherichia coli, K88, K99, 987P, F41, Fimbirial antigen, High density fermentation

摘要:

采用高密度发酵和普通深层通气两种方法培养含K88、K99、987P、F41菌毛抗原的四株猪埃希氏大肠杆菌，对其培养液进行活菌数（CFU）、OD值、pH值、RIHA效价的测定。实验结果表明，运用高密度发酵方法培养，各菌活菌数可达4.1～4.9?010/mL，RIHA效价为211～213；运用普通深层通气方法培养，各菌活菌数为0.51～0.59?010/mL，RIHA效价为24～25，可选择高密度发酵方法替代普通深层通气方法培养，用于制备猪埃希氏大肠杆菌K88、K99、987P、F41四价菌毛提纯苗。

Abstract:

Culture Escherichia coli fimbiral antigen K88，K99，987P AND F41 separately by bioreactor and culture-bottles, and then measure their CFU, OD, pH and RIHA. The experimental results show that CFU reached 4.1～4.9?010/mL and RIHA reached 211～213 when bacteria were cultured by bioreactor. CFU reached 0.51～0.59?010/mL and RIHA reached 24～25 when bacteria were cultured by culture-bottles. The results suggest that bioreactor can be used to culture Escherichia coli fimbirial antigen K88, K99, 987P, F41.