抗狂犬病毒单克隆抗体的纯化及标记

Purification and application of the Rabies viral monoclonal antibody

关键词:  狂犬病毒  单克隆抗体  荧光标记抗体

Key words:  Rabies virus  McAbs  Fluorescein-labeled antibodies  

摘要:

摘要：本实验对一株稳定、特异、高效的单克隆抗体(mAb)，进行了纯化和荧光素（FITC）的标记，利用标记产物与已有的抗狂犬病毒核蛋白单克隆抗体标记物相比较，效果良好，二者可以结合（或单独）使用；并且应用标记好的荧光抗体与FITC-抗狂犬病毒免疫球蛋白比较进行血清样品效价测定实验，来确定纯化、标记后的单克隆抗体的灵敏度，结果表明本实验制备的单克隆抗体与FITC-抗狂犬病毒免疫球蛋白具有相同的灵敏度：该单克隆抗体测得的血清样品效价结果与FITC-抗狂犬病毒免疫球蛋白测得的结果相同。因此，此荧光抗体可应用于狂犬病病毒检测（FAT）、病毒毒力测定（TCID50）、中和抗体实验（FAVN）等方面的检测实验；为狂犬病的相关研究提供有力的保障。

Abstract:

Abstract：The aim of this research is to purification a stabile, special and efficient monoclonal antibody(mAb)；used mark product with existing resistance resistance of the rabies virus nucleoprotein monoclonal antibody markers compared, and the results is good, both can ues combine(or separate); comparison on serum sample titer test to determine the purification and mark after the sensitivity of monoclonal antibodies by used marked fluorescent antibody and FITC making-implementing the rabies virus immunoglobulin, and the results show that the experiment of the preparation of monoclonal antibodies with FITC making-implementing the rabies virus immunoglobulin have the same sensitivity; Thus the fluorescent antibody can be applied to the rabies virus detection (FAT), virus poisonous force measurement (TCID50) and A.granoff experiment (FAVN) and so on，provide strong guarantee for rabies studies.