恒温扩增技术在动物疫病病原检测中应用现状及展望

Current status and prospects of Isothermal amplification in thedetection pathogens of animal disease

关键词:  恒温扩增  病原检测  展望

Key words:  Isothermal amplification  Pathogen Detection  Prospects

摘要:

文章介绍了链置换扩增技术、转录介导扩增技术、核酸序列依赖性扩增技术、环介导等温扩增技术、赖解旋酶恒温基因扩增技术、滚环扩增等六种恒温扩增核酸的技术的特点及其研究应用概况，并对恒温扩增技术的前景予以展望，以期为这些技术的应用提供参考。

Abstract:

In the paper, an overview of the current status relating to Isothermal amplifications including Strand Displacement Amplification, Transcription mediated amplification, Nucleic acid sequence-based amplification, Loop-mediated isothermal amplification, Helicase-dependant amplification and Rolling circle amplification were reviewed, and the features, applications, the challenges and the techniques problems of them were discussed . The prospects and further applications of Isothermal amplifications in their future development were considered,too.