猪肉发光细菌(PB-CS-01)的鉴定与生物学研究

identification and biology study of a photobacterium（ PB-CS-01 ）causing glowing pork

关键词:  发光细菌 细菌鉴定 16S rRNA基因序列分析 药敏实验 急性致病性实验

Key words:  Photobacterium phosphoreum, identification,16S rRNA sequence analysis, drug-sensitive test, acute pathogenic assay

摘要:

对一株从发光猪肉中分离到的发光细菌(PB-CS-01)进行了形态结构与培养特征的观察、生化特征研究以及16S rRNA基因序列分析。结果表明：该菌为革兰氏阴性球杆菌,在温度为18-22 癈及盐浓度为1.5-3.0%的培养基中生长良好，在光照好氧及厌氧条件下都能生长，发蓝绿色光。16S rRNA基因序列分析表明该菌(PB-CS-01)与GenBank中Photobacterium phosphoreum的同源性水平达100％，结合菌株的形态学和生化学特性，鉴定为Photobacterium phosphoreum。该菌对小鼠无急性致病性；对链霉素，复方新诺明和头孢呋辛等多种抗菌药物敏感；新洁尔灭、戊二醛等常用消毒剂能杀死该菌。

Abstract:

A photobaterium strain named PB-CS-01 was isolated from commercially available glowing pork and the biological identification and characterization was also performed. Strain PB-CS-01 was characterized as a Gram-negative bacterium that could be best grown in agar medium supplemented with 1.5%-3.0% salt at 18 癈-22 癈, and it can also grow well in aerobic conditions with light as well as in anaerobic conditions. Interestingly, this bacterium can given blue-green light. The PB-CS-01 was identified as Photobacterium phosphoreum, according to its colony morphological properties, biochemical properties, and the 16S rRNA sequence analysis. Pathogenic assay and drug-sensitivity test indicated that PB-CS-01 has no acute pathogenicity to mouse.and highly sensitive to antibiotics, such as streptomycin, cotrimoxazole, cefuroxime, etc.