微波诱变结合消泡剂定向选育VB12高产菌株的应用

Microwave Induced by Combining Antifoaming agent Directional

关键词:  微波诱变  消泡剂  脱氮假单胞杆菌  发酵

Key words:  microwave mutagenesis  antifoaming agent  nitrogen false aeromonas  fermentation

摘要:

运用微波诱变结合消泡剂定向选育VB12生产菌株—脱氮假单胞杆菌。结果表明：当微波功率800W、脉冲频率2450MHz，分离平皿中消泡剂加量3‰时，诱变剂量6s效果最明显。得到遗传稳定的突变菌株VB12Ⅰ09-1023#，通过摇瓶测得该突变株发酵单位高达268mg/L，比出发菌株提高了28.8%。120m3发酵罐试验，25批平均发酵单位高于对照7.5%。

Abstract:

Using microwave induced by combining antifoaming agent directional selection VB12 production pseudomonas denitrificans.The result shows that: when microwave power 2450MHz 800W, pulse frequency, separation AGAR disinfection defoamer quantity 3 per 1,000, mutagenic effect is the most obvious. Dose 6s Get VB12 strains of mutation genetic stability Ⅰ 09-1023 #, measured by the wave bottle fermentation unit as mutations 268mg/L, improved strains of 28.8%. 120m3 fermentation tests, an average of 25 batch fermented unit ck 7.5%.