| 摘要: |
| 为了检测高效表达鸡马立克氏病病毒gB基因的重组鸡痘病毒（rFPV-gB/R）的纯净性，我们对纯化后的0代重组病毒毒种进行了细菌和支原体检验；对中间试制的冻干疫苗在-20℃保存期进行了测定。结果表明，重组病毒毒种高度纯净，没有污染任何细菌或支原体，符合新制品制造和检验规程申报的要求；中试冻干疫苗在-20℃保存了12个月，病毒含量始终大于109PFU/ml，疫苗保存期至少为12个月。 |
| 关键词: 鸡马立克氏病 gB基因 重组鸡痘病毒 纯净性检验 保存期测定 |
| DOI： |
| 投稿时间：2010-06-28修订日期：2010-08-04 |
| 基金项目: |
|
| Purity Assay of the Original Recombiant Fowlpox Virus and the Valid Storage Time of the Pilot Product of the Lyophilized rFPV-gB/R Vaccine |
| lujun |
| (Shanghai Animal Disease Control Center) |
| Abstract: |
| A purity assay was performed with the original recombiant fowlpox virus and the pilot product of the lyophilized rFPV-gB/R vaccine. The results showed that rFPV-gB/R was free from exogenous bacterial and mycoplasma. The experiment for determining the valid storage time of the vaccine showed that the vaccine product remained effective after 12 months preservation at -20℃. |
| Key words: Marek’s disease gB gene recombinant fowlpox virus purity assay valid storage time |
