| 摘要: |
| 本文建立了高效液相色谱法(HPLC) 测定“甘草颗粒”中甘草酸的含量的检测方法。以甲醇-0.2mol/L醋酸铵溶液-冰醋酸（67:33:1）为流动相，检测波长为250nm。样品采取流动相超声提取，高效液相色谱分离，紫外检测器检测，外标法定量。结果甘草酸在0.002mg/ml～1.000mg/ml的浓度范围内拥有良好线性，相关系数为0.9999711。添加回收实验平均回收率为99.3%，回收率高。以浓度为0.20mg/ml标准溶液进行重复性实验，重复进样6次峰面积RSD为0.097%，重复性好。本法简便、快速，适用于 “甘草颗粒”中甘草酸的含量，分离效果较好、回收率高，稳定性好。 |
| 关键词: 甘草颗粒、甘草酸、含量、高效液相色谱法 |
| DOI： |
| 投稿时间：2010-06-10修订日期：2010-09-23 |
| 基金项目: |
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| Determination of Glycyrrhizae acid in the Granulae Glycyrrhizae by HPLC |
| luoyang |
| (Guizhou Province animal medicine supervision place) |
| Abstract: |
| A high performance liquid chromatogramphic ( HPLC) method was developed for the determination of glycyrrhizae acid in the granulae glycyrrhizae . The sample was separated with a mobile phase of methanol-0.2mol/L ammonium acetate solution-acetic acid (67 ∶33 ∶1). Detection wavelength was 250 nm . The linear range was0.002mg/ml～1.000mg/ml ,correlation coefficient was0.9999711. The recovery was 99.3%. The analytical result s showed that the method was rapid , simple , and easy to operate. |
| Key words: Granulae Glycyrrhizae,Glycyrrhizae Acid ,Content,High Performance Liquid Chromatography（HPLC） |
