鸡蛋中氟喹诺酮类药物残留量检测方法研究

Study on Detection Method of Quinolones Residues in Egg

关键词:  高效液相色谱法  氟喹诺酮类药物  残留  鸡蛋

Key words:  HPLC  quinolones  residues  egg

摘要:

建立一种可同时检测鸡蛋中四种氟喹诺酮类药物(环丙沙星、达氟沙星、恩诺沙星和沙拉沙星)残留的高效液相色谱—荧光检测法。结果表明四种氟喹诺酮类药物中环丙沙星、恩诺沙星、沙拉沙星在2~500 ng/mL呈良好的线性关系，R2均大于0.9999，达氟沙星在0.4~100 ng/mL呈良好的线性关系，R2大于0.9999。环丙沙星、恩诺沙星、沙拉沙星的定量限为10 µg/kg，达氟沙星的定量限为2 µg/kg。环丙沙星、恩诺沙星、沙拉沙星在10~200 µg/kg的浓度添加水平上，其回收率在70%~100%，达氟沙星在2~40 µg/kg的浓度添加水平上，其回收率在70%~100%，批内、批间的相对标准偏差均小于10%。

Abstract:

A method for determination of quinolones residues in egg with HPLC by fluorescence detector detection was established. A good linearity of the calibration curves for Ciprofloxacin、Enrofloxacin、Sarafloxacin was obtained with the range of 2-500ng/mL （r>0.999），and Danofloxacin was obtained with the range of 0.4-100ng/mL （r>0.999）. The limit of quantification of Ciprofloxacin、Enrofloxacin、Sarafloxacin was 10 ng/g ，and Danofloxacin was 2 ng/g . The average recoveries of Ciprofloxacin、Enrofloxacin、Sarafloxacin from spiked eggs at three concentrations of 10, 50, 200ng/g ranged from 70 % to 100%(n=5)，and Danofloxacin from spiked eggs at three concentrations of 2, 10, 40ng/g ranged from 70% to 100%(n=5). The intra- and inter-batch variation coefficients were both less than 10%.