反相高效液相色谱法测定硝碘酚腈含量

Quantity study of Nitroxynil by RP-HPLC

关键词:  硝碘酚腈  反相高效液相色谱法  含量测定

Key words:  Nitroxynil  RP-HPLC method  Assay

摘要:

采用反相高效液相色谱法（RP-HPLC）测定了硝碘酚腈含量。以乙腈-0.01 mol/L磷酸盐pH6.5 (20:80，V/V)为流动相，Agilent Zorbax SB-C18色谱柱 (4.6mm×250 mm，5 μm)；流速1.0 mL/min；柱温25℃；检测波长273 nm。结果表明：在0.050～1.20 mg/mL范围内硝碘酚腈浓度与峰面积呈良好线性关系(r=0.9995)，最低检出浓度为2.5 ng/mL，最低定量浓度为5.0 ng/mL 。低、中、高3种浓度回收率分别为100.21%，99.07%，101.90%，RSD=0.48%（n=9）。本方法专属性强、准确、简便、灵敏度高，结果稳定，可用于硝碘酚腈的含量测定。

Abstract:

Abstract: Nitroxynil was determined by a RP-HPLC method. The method was performed with a Agilent Zorbax SB-C18 column (4.6mm×250 mm,5 μm) at 25 ℃, and the mobile phase was acetonitrile-0.01 mol/L phosphate pH6.5 (20:80,V/V). The flow rate was 1.0 mL/min. The detection wavelength was 273 nm. The result shows that there is a good linear relationship(r=0.9995) between peak area and nitroxynil concentration at the range of 0.050~1.20 mg/mL, the limit of detection was 2.5 ng/mL, and the limit of quantitation was 5.0 ng/mL, and the method recoveries for low, medium and high were 100.21%, 99.07% and 101.90%, RSD=0.48% (n=9). The method is selective, simple and suitable for Assay of nitroxynil sample.