| 摘要: |
| 用eri作为布鲁氏菌属特异性基因，以IS711基因拷贝数差异作为布鲁氏菌种间特异性标志，建立了鉴别牛、羊、猪种布鲁氏菌株的多重PCR方法。结果：牛种布鲁氏菌2308株扩增出大小为494 bp和178 bp的两条带，羊种布鲁氏菌M28株扩增出大小为733 bp和178 bp的两条带，猪种布鲁氏菌S1330株扩增出大小为285 bp和178 bp的两条带，均与预期吻合；而胸膜肺炎放线杆菌、多杀性巴氏杆菌、流产沙门菌、都柏林沙门菌、大肠杆菌均未扩增出任何条带。硫化氢和血清学试验结果也符合相应种布鲁氏菌的特点。结果表明，本研究的多重PCR方法可用于牛、羊、猪种布鲁氏菌株的快速鉴定。
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| 关键词: 布鲁氏菌 eri基因 IS711基因 多重PCR |
| DOI： |
| 投稿时间：2009-11-05修订日期：2009-12-16 |
| 基金项目:科技基础性工作专项（2008FY130100）：重大动物疫病病原相关制品标准物质研究 |
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| A Muti-PCR Assay for Differentiating the Strains of B.abortus, B.melitensis and B.suis |
| pengxiaobing |
| (China Institue of Veterinary Drug Control) |
| Abstract: |
| A multi-PCR assay was developed to differentiate the strains of B.abortus, B.melitensis and B.suis on basis of eri and IS711 genes. Strains of B.abortus 2308, B.melitensis M28 and B.suis S1330 could be differentiated by this assay successfully. Sizes of 494, 178 bp were amplified from 2308, and 733, 178 bp from M28, and 285, 178 bp from S1330. No specific bands was amplified from the control bacterias such as Actinobacillus pleuropneumoniae, Pasteurella multocida, Salmonella abortus, Salmonella dublin and E.coli. Futhermore, the specificity of PCR was confirmed by sulfureted hydrogen test and serological identification. The result indicated that multi-PCR could be a rapid way to differentiate the strains of B.abortus, B.melitensis and B.suis. |
| Key words: Brucella eri gene IS711 gene multi-PCR |
