紫锥菊提取物的Ames试验

Study of Ames Test of Echinacea Herb P.E

关键词:  紫锥菊提取物  Ames致突变试验

Key words:  Echinacea Herb P.E  Ames test

摘要:

通过对紫锥菊提取物进行鼠伤寒沙门氏菌回复突变试验，评价该材料的潜在致突变性。用紫锥菊提取物制备混悬液进行Ames试验，采用平板掺入法,计数TA97，TA98，TA100，TA102标准测试菌株在4个不同浓度下，于37 ℃培养48 h后的回复突变菌落数。结果显示无论在（+）S9 和（-）S9 的情况下,各剂量组均未引起测试菌株回变菌落数的明显增加，Ames试验结果为阴性。说明紫锥菊提取物不引起鼠伤寒门菌的回复突变数增加，无致基因突变性。

Abstract:

The experiment was conducted with a view to evaluate the potential mutagenicity of Echinacea Herb Purpurea. Echinacea Herb Purpurea was produced to diffusion and the diffusion was tested through Ames test.In the flat-plate incorporation test of salmonella typhimurium , the average number of spontaneous revertants of TA97, TA98, TAl00, and TA102 at four concentrations was calculated after incubation at 37 ℃ for 48 hours.The results showed that whether adding S 9 or not,the number of induced revertants did not increase significantly compared to that of spontaneous revertants. The Ames test was negative.It indicated that Echinacea Herb Purpurea did not cause the increase of the back mutation in murine typhoid salmonella and could not cause gene mutation.