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紫外分光光度法测定硫酸庆大霉素注射液含量方法的建立
冯学忠
0
(广东大华农动物保健品股份有限公司)
摘要:
以水为参比液,采用紫外分光光度法在232nm波长处测定硫酸庆大霉素注射液中庆大霉素的含量。实验结果表明,庆大霉素在10U/ml~60U/ml浓度范围内,其吸光度与浓度呈良好的线性关系,相关系数r=0.9998,平均回收率为99.2%,RSD为0.8%(n=6)。该方法操作简便、快速、结果准确,适于作为生产企业中间产品的质量控制方法。
关键词:  硫酸庆大霉素  注射液  紫外分光光度法  含量测定
DOI:
投稿时间:2009-08-24修订日期:2009-09-11
基金项目:
Determination of Gentamycin Sulfate Injection Content by UV Spectrophotometry
fengxuezhong
(Guangdong Dahuanong Animal Health Products Co.Ltd)
Abstract:
An UV spectrophotometry method for the determination of gentamycin content in gentamycin sulfate injection was established with water as reference solution at 232nm.The calibration curve for gentamycin was linear within the concentration range of 10U/ml~60U/ml (r=0.9998).The average recovery rate was 99.2%,and the RSD was 0.8%(n=6).This method was proved to be simple,rapid and precise,and can be used for the quality control of gentamycin sulfate injection.
Key words:  gentamycin sulfate  injection  UV spectrophotometry  content determination

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