中兽药制剂中喹噁啉类药物检测方法的研究

Development of detection of quinoxalines in Chinese veterinary medicine by HPLC

关键词:  关键词：中兽药  喹噁啉  高效液相色谱法  

Key words:  Keywards: Chinese veterinary medicine, quinoxalines, HPLC

摘要:

摘要 建立中兽药制剂中喹乙醇、乙酰甲喹、卡巴氧、喹烯酮药物的测定。样品经三氯甲烷:甲醇（3：1）提取后，苯基柱分离，在360nm处检测。四种喹噁啉类药物在0.2μg/ml～100μg/ml浓度范围内呈线性相关。在空白样品中添加50mg/kg～150mg/kg浓度，回收率范围在87.90%～96.82%之间，批内变异系数为0.55%～4.65%。本方法的检测限为20mg/kg，定量限为50mg/kg。结果表明，本方法适合中兽药制剂中喹噁啉类药物的测定。 关键词：中兽药；喹噁啉类药物；高效液相色谱法；

Abstract:

Detection of quinoxalines in Chinese veterinary medicine by HPLC Jia Zhenming,Wu Ningpeng, Song Zhichao, Guo Furong, Chen Qiang Institute of Henan veterinary drug contral, Zhengzhou, 450008 Abstract: A method for detection of olaquindox,maquindox,carbadox,quinocetone in Chinese veterinary medicine by HPLC was developed. The samples were extracted by chloroform:methanol(3:1) , and separeted by phenyl cloumn, detected by ultraviolet detector at 360nm. The four of quinoxalines were lineary in the range of 0.2μg/ml～100μg/ml .In blank samples fortified at 50mg/kg～150mg/kg,the average recoveries ranged from 87.90%～96.82%, with the coefficients of variation within-day of 0.55%～4.65%. The limit of detection was 20mg/kg,and the limit of quantitation was 50mg/kg.The results showed that the method is suitable for detection of quinoxalines in Chinese veterinary medicine. Keywards: Chinese veterinary medicine, quinoxalines, HPLC