重组溶菌酶基因工程质粒治疗奶牛乳房炎的效果分析

ANALYSIS OF TREATING DAIRY COW MASTITIS USING RECOMBINANT PLASMID CONTAINING LYSOZYME GENE

关键词:  奶牛乳房炎，重组质粒pcDNAKLYZ，抗生素，细菌计数

Key words:  Dairy cow mastitis, recombinant plasmid pcDNAKLYZ, antibiotics, bacteria count

摘要:

本实验通过对上海市光明荷斯坦金山种奶牛场采集的临床型和隐性乳房炎患牛的94份奶样进行细菌培养，在注射抗生素的30份奶牛乳样的培养结果中，阴性率为16.67%（5/30），阳性率为83.33%(25/30) ，菌落计数结果均值为874.93；在注射人溶菌酶的重组质粒pcDNAKLYZ的64份奶牛乳样的培养结果中，阴性率为35.94%（23/64），阳性率为64.06%（41/64），菌落计数结果均值为303.75。经过统计学分析，重组质粒组的计数结果均值显著小于抗生素组的计数结果（P<0.05）。从结果综合来看，重组质粒的抑菌效果显著高于抗生素的抑菌效果，该重组质粒对奶牛乳房炎具有较好的治疗效果。

Abstract:

In this experiment，we make several bacterial culture with 94 parts of milk sample collected from cattle infected with subclinical or clinical mastitis. Among 30 parts of culture of antibiotic group, negative result accounts for 16.67%（5/30）, positive result accounts for 83.33%(25/30), the mean clump count result is 874.93. Among 64 parts of culture of recombinant plasmid group, negative result accounts for 35.94%（23/64）, positive result accounts for 64.06%（41/64）, the mean clump count result is 303.75. The mean clump count result of recombinant plasmid group is significantly lower than that of antibiotics group（P<0.05）.In general, from the result of clump count, bacteriostasis of recombinant plasmid is more effective and recombinant plasmid has a better treatment effect on the cow mastitis.