GC/MS法研究猪体内盐酸克伦特罗的残留代谢规律

Studies on the Residue Elimination of clenbuterol in pig Using GC/MS

关键词:  猪  盐酸克伦特罗  残留  代谢  气相色谱-质谱法（GC/MS）

Key words:  pig  clenbuterol  residual  elimination  gas chromatography – mass spectrometry（GC/MS）

摘要:

样品用乙酸乙酯提取，盐酸溶液萃取后，再经SCX固相萃取柱净化，50℃水浴中氮气吹干后衍生化。以气相色谱－质谱选择86.212.262.277四个碎片离子进行定性定量分析。盐酸克伦特罗在0.01mg/L～2.0 mg/L成线性（R2为0.999），猪肉中3种不同添加浓度0.01、0.10和0.20mg/kg下盐酸克伦特罗平均回收率分别为88.31%、92.85%、93.97%，相对标准偏差分别为5.26%、7.58%、4.59%，样品中最低检测限（S/N=3）为0.1ug/kg。应用该方法研究了盐酸克伦特罗在猪体内的代谢并初步探讨其代谢机理。结果表明：在饲喂浓度(3 mg／kg)一个月后，猪体内盐酸克伦特罗蓄积量不算太高，最高蓄积浓度为493.59ug／kg，而且停药后代谢又非常快

Abstract:

The clenbuterole was extracted from samples by employing ethyl acetate and hydrochloric acid solution，the cleaning procedure was performed by SCX solid phase extraction cartridge．The extract was warmed with water at 50 ℃ and blowed with nitrogen until it got near to dryness.Then it was derivated．Retention time and specific ions were used to identify melamine．The selective fragments were m/z 86.212.262.277，respectively．The calibration curve of clenbuterol was linear (correlation coefficient：0.999) within the range of 0.01mg/L～2.0 mg/L．The average recoveries (at 0.01mg/kg、0.10 mg/kg and 0.20 mg/kg) were 88.31%，92.85%，93.97%．The relative standard deviations (RSD) of the method was <8% for each case，and the limit of detection (LOD)，defined in terms of 3 times baseline nosie was 0.1ug/kg．respectively．The developing method was applied to study the metabolize behavior and mechanism of clenbuterol in pig．The result indicated that the residual of melamine was found in pig while starting to feed， the content of clenbuterol in pig was increased gradually，and the highest content of clenbuterol was 493.59ug／kg，but the content of clenbuterol in pig was reduced gradually immediately while stoping feed