高效液相色谱法测定环丙氨嗪的含量

Determination of Cyromazine by HPLC

关键词:  高效液相色谱法  环丙氨嗪  含量测定

Key words:  HPLC  Cyromazine  assay

摘要:

建立了环丙氨嗪含量测定的高效液相色谱方法。采用Phenomenex Prodigy C18色谱柱(250×4.6 mm, 5m),甲醇：乙腈：缓冲液(每1000 ml水中加3.72 g磷酸氢二钾和6.48 g磷酸二氢钾)=5:2:93为流动相，流速为2.0 ml/min，检测波长214 nm，柱温20℃。该方法线性范围为0.00799~0.01229 mg/ml，r=0.99，系统精密度RSD=0.25% (n=6)，平均回收率为99.2%(RSD=0.82%)，可用于环丙氨嗪的含量测定。

Abstract:

The HPLC method was established for the determination of cyromazine. A chromatographic column of Phenomenex Prodigy C18(250×4.6 mm, 5 m) was used, with the mobile phase of methanol-acetonitrile-buffer solution (5:2:93) (every 1000 ml water add 3.72 g dipotassium hydrogen phosphate and 6.48g potassium dihydrogen) at the folw rate of 2.0 mLmin-1 and the detection wavelength of 214nm. The linear concentration was within the range of 0.00799~0.01229 mg/ml(r=0.99), RSD of system precision was 0.25% (n=6), and the average recovery was 99.2%(RSD=0.82%). This method was suitable to determine the content of cyromazine.