支原体检验用培养基的研究

The study of the medium in the tests for absence of Mycoplamas

关键词:  改良Frey培养基和支原体培养基、灵敏度、反复冻融、最小检出量、有效期

Key words:  Modified Frey medium and Mycoplamas medium,sensitivity,freezing and thawing,limit test,expire date

摘要:

通过水质和血清对比试验，结果表明：以水质电导率低于0.5µs/cm；猪血清总蛋白不低于49g/L，胆固醇不高于1.66mmol/L为标准，制备的改良Frey培养基和支原体培养基活菌滴度均达109CCU／ml。对滑液支原体GX11-T株及猪鼻支原体BTS-7 株1～5代对数生长期培养物检测，均达109CCU／ml。改良Frey培养基与支原体培养基经反复冻融8次；或改良Frey培养基经37℃保存24小时； 支原体培养基37℃保存8小时均不影响灵敏度。改良Frey培养基在鸡胚成纤维细胞及鸡胚尿囊液中，最小检测量分别为2CCU/ml和4CCU/ml；支原体培养基在BHK21细胞中最小检测量为8CCU/ml。改良Frey培养基和支原体培养基4℃有效期为1月，－20℃为12月。

Abstract:

The result of different kinds of water and serum is confirmed that the tires of medium made by the conductivity rate of water with not more than 0.5Чs/cm 、general protein of serum with not less than 49 g/L and cholesterol with not higher than 1.66mmol/L reaches 109CCU／ml。1～5 logarithm phase culture of Mycoplamas gallisepticum and Mycoplamas synoviae is attained 109CCU／ml by detection with medium。After 8 times by repeating freezing and thawing between modified Frey medium and Mycoplamas medium,or modified Frey medium in 37℃ for 24 hours and Mycoplamas medium in 37℃ for 8 hours ,the sensitivity of medium retains invariability. the limit tests for modified Frey medium is 2CCU/ml and 4CCU/ml in allantoic fluid of chick embryo and chicken embryo fibroblasts respectively.and the limit tests for Mycoplamas medium is 8CCU/ml in BHK21 cell lines.the expire date between modified Frey medium and Mycoplamas medium is 1 month in 4℃,or 12 months in －20℃.