| 摘要: |
| 建立中兽药散剂白头翁散中盐酸小檗碱的含量测定方法，为其质量标准的制订提供实验依据。采用HPLC法测定，色谱条件：Eclipse XDB-C18柱(5µm,4.6mm×150mm)；流速：1.0mL/min；流动相：乙腈︰0.05mol/L磷酸二氢钾=28：72；柱温：30℃；检测波长：348nm。盐酸小檗碱在进样量为0.05～0.50µg内呈良好的线性关系， r=0.9999；样品中盐酸小檗碱的平均含量为0.781%，RSD为0.86%。该方法简便，易行，专属性强，可以作为白头翁散中盐酸小檗碱的含量测定方法。 |
| 关键词: 白头翁散 盐酸小檗碱 HPLC 含量测定 |
| DOI： |
| 投稿时间：2009-02-22修订日期：2009-03-10 |
| 基金项目: |
|
| Determination of the Content of Berberine Hydrochloride in Baitouweng Powder by HPLC |
| LIUJIANHUA |
| (Engineering College of Animal Husbandry and Veterinary Science，Henan Agricultural University) |
| Abstract: |
| To determine the content of Berberine Hydrochloride in Baitouweng Powder and to provide evidence for its quality criteria. A HPLC method was established as follows: Eclipse XDB-C18 Column (4.6mm×150mm,5µm)；Acetonitrile:0.05mol/L KH2PO4 =28：72 as Mobile phase, flow rate at 1.0mL/min and detection wavelength at 348nm. The calibration curve of Berberine Hydrochloride was linear in the range of 0. 05 -0.50μg with the correlation coefficient of 0.9999. The average content of Berberine Hydrochloride in Baitouweng Powder was 0.781%,and RSD=0.86%. This method is simple,accurate and repeatable , and can be used for the quality of Baitouweng Powder. |
| Key words: Baitouweng Powder Berberine Hydrochloride HPLC content determination |
