| 摘要: |
| 建立了测定氟苯尼考悬浮乳剂含量的高效液相色谱法（HPLC）。采用C18色谱柱(4.6mm×250mm,5μm),以乙腈-水(25:75)为流动相,流速1.0mL/min,检测波长223nm,进样量20μL,柱温为室温；氟苯尼考浓度在40～60μg/ml时,其峰面积与浓度的线性关系良好(r=0.9999)；无杂质干扰，平均回收率为100.18%,平均批内变异系数为0.82%，批间变异系数为0.22%。本方法操作简便,分析快速,结果准确,适用于氟苯尼考悬浮乳剂的含量测定。 |
| 关键词: 氟苯尼考悬浮乳剂 含量 高效液相色谱法 |
| DOI： |
| 投稿时间：2009-02-03修订日期：2009-03-16 |
| 基金项目:十一五国家科技支撑计划“兽用抗菌药物新制剂的研制与应用”（2006BAD31B06）；上海市科技兴农重点攻关项目“兽用抗菌药物长效注射剂的研制”（沪农科攻字（2006）第10－3号） |
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| Content Determination of Florfenicol Suspoemulsions by HPLC |
| weihaitao |
| (College of Veterinary Medicine, South China Agricultural University) |
| Abstract: |
| A method for the content determination of Florfenicol Suspoemulsions by HPLC was established. The HPLC system consisted of C18 column (4.6mm×250mm,5μm),acetonitrile-water (25∶75) as mobile phase, the flow rate was 1.0mL/min,detection wavelength was 223nm,the injection volume was 20μL and column temperature was room temperature. There was no interference occurred from impurities.The calibration curve was linear in the range of 40～60μg/mL(r=0.9999),the average recovery was 100.18%,the average intraassay coefficient of variation was 0.82%,the average interassay coefficient of variation was 0.22%.The method is rapid,simple and accurate for the content determination of Florfenicol Suspoemulsions. |
| Key words: Florfenicol Suspoemulsions Content HPLC |
