| 摘要: |
| 摘要：目的 建立露水草提取物的质量标准。方法 用紫外分光光度法，在242nm±2nm处进行最大吸收的鉴别；用薄层色谱法对其主要成分β－蜕皮激素进行鉴别；用高效液相色谱法测定露水草提取物中β－蜕皮激素的含量，流动相为乙腈－甲醇－水（1 ：2 ：4），流速为1mL/min，检测波长为243nm，柱温为35℃，理论塔板数不低于2500。结果薄层色谱无干扰，专属性强；β－蜕皮激素质量浓度在0.6～4.2mg/mL范围内与峰面积线性关系良好，r＝0.99997，加样回收率为99.7％，RSD为0.6％（n＝6）。结论 所用方法简便、准确、重现性好，可用于露水草提取物的质量控制。 |
| 关键词: 露水草提取物 β－蜕皮激素 高效液相色谱法 质量标准 |
| DOI： |
| 投稿时间：2008-12-02修订日期：2009-04-21 |
| 基金项目: |
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| Quality Standard for Extractum Cyanotis Vaga Siccus |
| tanmei |
| (Yunnan Institute of Veterinary Drug Control) |
| Abstract: |
| Abstract : Objective To establish the quality standard for Extractum Cyanotis Vaga siccus .Methods beta-Hydroxyecdysone were indentified by TLC,and beta-Hydroxyecdysone was determined by HPLC. Results beta-Hydroxyecdysone could be indentified by TLC. The linear range of beta-Hydroxyecdysone was 0.6～4.2mg/mL (r=0.99997).The recovery rate was 0.2% (n=7). Conclusion This method is simple, accurate, with high specificity and good repeatability. It can be used for the quality control of Extractum Cyanotis Vaga siccus. |
| Key words: Extractum Cyanotis Vaga siccus, beta-Hydroxyecdysone, HPLC, quality standard |
