RP-HPLC法测定博落回总碱中白屈菜红碱含量的方法研究

Research on the Method for Determination of Chelerythrine in Total Alkaloid of Macleaya Cordata by RP-HPLC

关键词:  白屈菜红碱  博落回总碱  RP-HPLC

Key words:  Chelerythrine  Total Alkaloid of Macleaya Cordata  RP-HPLC

摘要:

目的 建立博落回总碱中白屈菜红碱的RP-HPLC含量测定方法。方法 采用 日本资生堂ODS-MG C18液相色谱柱（4.6mm×150mm，5µm）；以乙腈-0.1%磷酸溶液（45∶55）（每100mL加十二烷基磺酸钠0.1g）为流动相；检测波长270nm；流速为1.0mL•min-1。结果 白屈菜红碱峰形对称，拖尾因子低于1.5；在0.1µg～0.9µg范围内峰面积与浓度呈良好的线性关系(r=0.9999，n=5)；在80％、100％、120％三种添加水平下的平均回收率分别为97.4%（RSD=0.7％，n=3）、98.6% （RSD=0.1％，n=3）、99.5%（RSD=0.8％，n=3）。结论 利用该法测定博落回总碱中的白屈菜红碱，结果准确度高、精密度好，可作为博落回总碱的质量控制方法。

Abstract:

Objective To develop an RP-HPLC method for the determination of Chelerythrine in total Alkaloid of Macleaya Cordata. Methods The Shiseido ODS-MG C18 column (4.6mm×150mm, 5µm) was used with acetonitrile-0.1% phosphoric acid solution (45∶55) (0.1g Sodium dodecyl sulfonate in 100mL solution) as mobile phase. The detection wavelength was 270nm. The flow rate was 1.0mL•min-1. Results A symmetrical peak of Chelerythrine was obtained and the tailing factor was less than 1.5. The linearity range was 0.1µg～0.9µg (r=0.9999，n=5) and under 80%,100%,120% level of accession, the average recoveries of Chelerythrine were respectively 97.4% (RSD=0.7％，n=3), 98.6% (RSD=0.1％，n=3), 99.5% ( RSD=0.8％，n=3). Conclusion The method is sensitive and reliable and can be used as a quality control method for the Chelerythrine in total Alkaloid of Macleaya Cordata.