高效液相色谱法测定癸氧喹酯溶液的含量

HPLC determination of decoquinate solution

关键词:  癸氧喹酯  高效液相色谱法  含量测定

Key words:  decoquinate  HPLC  assay

摘要:

摘要 建立了癸氧喹酯溶液含量测定的高效液相色谱方法。采用Zorbax Eclipse C18色谱柱（4.6mm×150mm,5μm），以甲醇－乙腈－冰乙酸（45：55：0.1）溶液（用三乙胺调pH值至7.0，每1000mL再加入1.0g硝酸钙）为流动相，检测波长267nm，流速为1.0mL/min，外标法定量。该方法最低检测限为0.05mg/mL，在1～100µg/mL浓度范围内线性关系良好（r2＝0.9999），平均回收率99.67%，可用于癸氧喹酯溶液的含量测定。

Abstract:

Abstract A HPLC method has been established for the determination of decoquinate solution. A Zorbax Eclipse C18 column(4.6mm×150mm,5µm) was used with the mobile phase of methanol-acetonitrile- glacial acetic acid (45:55:0.1)( to adjust pH=7.0 by triethylamine and then adding 1g calcium nitrate per 1L mobile phase),at the detection wavelength of 267nm ,with a flow rate of 1.0 mL/min. The quantification was carried out by the external standard method. The limit of detection of the method was 0.05µg/mL. The calibration curves have good linear within 1-100 µg/mL (r2=0.9999). The average recovery of decoquinate was 99.67%. The all results were shown that the HPLC method may be used for the determination of decoquinate solution.