奶牛子宫内膜炎病原菌的分离鉴定及ε-多聚赖氨酸的抑菌作用

Isolation and identification of pathogenic bacteria from endometritis in dairy cows and bacteriostasis of ε-Polylysine

关键词:  ε-多聚赖氨酸  子宫内膜炎  抑菌作用

Key words:  ε-Polylysine  endometritis  bacteriostasis

摘要:

从26头子宫内膜炎患牛的子宫分泌物中检测到21个分泌物中有细菌，它们是凝固酶阴性葡萄球菌（coagulase negative staphylococci, CNS）（19.2%, 5/26)、牛链球菌（Streptococcus bovis） （15.4%，5/26）、化脓链球菌（Streptococcus pyogenes）（7.7%，2/26）、大肠杆菌（Escherichia coli）（15.4%，4/26）、普通变形杆菌(Proteus vulgaris）（30.8%，8/26）、肺炎克雷伯菌（Klebsiella pnenmoniae）（11.5%，3/26）；5个分泌物中未检测到细菌（19.2%，5/26）。药敏试验结果表明，多数病原菌对强力霉素和苯唑青霉素耐药，但对环丙沙星、庆大霉素、氧氟沙星敏感。试管稀释法结果表明，ε-多聚赖氨酸对子宫分离菌的最小抑菌浓度（MIC）为45 μg/ml～215μg/ml。由于ε- PL对奶牛子宫内膜炎致病菌良好的抗菌作用以及在食品中的安全性，有必要进一步研究ε- PL上对奶牛子宫内膜炎的治疗作用。

Abstract:

Twenty-one bacterial isolates were obtained from the 26 uterus secretions of dairy cows with endometritis. They were coagulase negative staphylococci （19.2%, 5/26) , Streptococcus bovis （15.4%，5/26）, Streptococcus pyogenes （7.7%，2/26）, Escherichia coli（15.4%，4/26）, Proteus vulgaris（30.8%，8/26）and Klebsiella pnenmoniae（11.5%，3/26）. Five samples had no bacteria detected（19.2%，5/26）. These isolates were resistant to doxycycline and oxacillin, but sensitive to ciprofloxacin, gentamicin and ofloxacin. In vitro test showed that the minimum inhibitory concentration of ε-Polylysine (ε-PL) to the bacterial isolates were 45 μg/ml～215μg/ml. ε-PL should be further evaluated for its therapeutic effect on endometritis in dairy cows due to its strong inhibitory effect on the endometritis-causing bacteria and safety in food.