鸡新城疫、传染性支气管炎病毒同胚接种试验

Test of Newcastle disease virus and infectious bronchitis virus inoculated

关键词:  鸡新城疫病毒  鸡传染性支气管炎病毒  同胚接种

Key words:  Newcastle disease virus  Infectious bronchitis virus  Inoculated in the same embryo

摘要:

通过对五个批次的无特定病原体(SPF)鸡胚进行鸡新城疫病毒（NDV）、鸡传染性支气管炎病毒（IBV）同胚接种，收获72～120 h死胚及120 h活胚尿囊液，分别测定NDV和IBV的病毒含量，结果表明NDV病毒含量达到了108.3～108.7EID50/0.1mL, IBV的病毒含量达到了106.3～106.5EID50/0.1mL。两种病毒含量均达到《中华人民共和国兽用生物制品制造及检验规程》（2000版）中鸡新城疫、传染性支气管炎二联活疫苗病毒含量要求的标准。表明该方法即可降低成本，又简化了生产工艺。

Abstract:

Newcastle disease virus and Infectious bronchitis virus were inoculated in the same SPF embryo for five groups. The allantoic fluid of the embryos which died in 72～120 hours or live in 120 hours were Harvested and then the embryo infectious dose (EID50) of NDV and IBV were detected respectively. The results indicated that the EID50 of NDV were 108.3～108.7/0.1mL and the EID50 of IBV were 106.3～106.5/0.1mL. The virus concentration of five groups allantoic fluids were reached the standard of Newcastle disease and Infectious bronchitis vaccine (living vaccine) which in The Manufacturing and Examination Regulations of the People's Republic of China used in animal vaccine (2000 versions). This test suggested that the two kinds of virus were cultured in same SPF embryo could reduce cost of vaccine manufacture and simplify technology of vaccine manufacture.