气相色谱－质谱法测定猪尿中莱克多巴胺

Determination of Ractopamine in Swine Urine by Gas Chromatography－Mass Spectrtometry

关键词:  气相色谱-质谱  猪尿  残留  莱克多巴胺

Key words:  Gas Chromatography－Mass Spectrometry  swine urine  residue  ractopamine

摘要:

[摘要]建立了猪尿中莱克多巴胺的气相色谱－质谱(GC／MS)检测方法。样品经β一盐酸葡萄糖醛苷酶/芳基硫酸酯酶水解，乙酸乙酯提取、盐酸溶液萃取、SCX固相萃取柱净化、N，O一双三甲基硅基－三氟乙酰胺(BSTFA)衍生化后进行气相色谱－质谱测定。结果表明，方法检出限为0.3μg／L、定量限为0.5 μg／L，线性范围为10～500μg/L。在三个添加浓度2µg/L、4µg/L、8µg/L水平上，莱克多巴胺的平均回收率为 85％～ 115％，批内、批间相对标准偏差< 10％。

Abstract:

Abstract： A gas chromatography-mass spectrometric (GC/MS) method has been established for the determination of ractopamine in swine urine．The samples were hydrolyzed by β－glucuronidase/arylsulfatase, extracted with ethyl acetate and hydrochloric acid solution，cleaned up on SCX cartridge, derived with N，O-bis(trimethylsily1) trifluoroacetamide(BSTFA)， and detected by GC/MS ．The results indicated that the limit of detection (LOD) of ractopamine in swine urine was 0.3 μg/L, the limit of quantification (LOQ) was 0.5 μg/L，and the linearity was within the range of 10～500μg/L．The mean recovery of swine urine samples fortified at three levels of 2µg/L, 4µg/L and 8µg/L was within the range of 85％～115％, and intra- and inter- batch relative standard deviation were less than 10％.