几株野生鸟类来源的Ⅰ型禽副粘病毒分子生物学特征的研究

The Molcular Biological Characterization of Avian paramyxoviruses TypeⅠ Isolated From Several Wild Birds

关键词:  Ⅰ型禽副粘病毒  F基因  序列分析  基因型

Key words:  Avian paramyxoviruses typeⅠ  F gene  Sequence analysis  genotype

摘要:

参考GenBank中的Ⅰ型禽副粘病毒（Avian paramyxovirus,APMV-1）序列，在F基因的3′端相对保守区设计1对引物，利用RT-PCR方法对华南农业大学兽医学院禽病学教研室近两年来从广州和深圳两个地区分离的4种动物来源共10株Ⅰ型禽副粘病毒毒株的F基因进行了扩增，其扩增长度约为450bp。测序结果表明：广州株与NDV的基因Ⅵ型有高度的同源性，1个深圳株与Ⅶ型有较高的同源性，其氨基酸序列均符合F蛋白裂解位点区（112－117）强毒株的氨基酸序列特征；其它4个深圳株均与基因Ⅰ型有很高的同源性，其氨基酸序列均符合F蛋白裂解位点区（112－117）弱毒株的氨基酸序列特征。

Abstract:

Ten field strains of Avian paramyxoviruses typeⅠ(APMV-1) were differentiated from 4 kinds of wild birds in Guangzhou and Shenzhen . A pair of primers specific to APMV-Ⅰ gene F 3’end were designed and synthesized according to the known sequences from GenBank, and were used to amplify of APMV-1 cDNA gene F 3′end by RT-PCR from total RNA, which is 450bp in length. The seqences of PCR products show that the strains isolated from Guangzhou belong to genetype Ⅵone strain isolated from Shenzhen belong to genetype VII, whose amino acid sequences of the cleavage site region ( 112－117) are similar to that of all other rulent strains and that the other four strains isolated from Shenzhen belong to genetypeⅠwhose amino acid sequences of the cleavage site region ( 112－117) are similar to that of all other rulent strains.