HPLC-MS/MS快速检测动物尿液中的莱克多巴胺和克伦特罗

Rapid Determination of Ractopamine and Clenbuterol in Animal urine with HPLC-MS/MS

关键词:  HPLC-MS/MS  动物尿液  莱克多巴胺  克伦特罗

Key words:  Ractopamine  Clenbuterol  Animal urine  HPLC-MS/MS

摘要:

建立了以高效液相色谱-质谱（HPLC-MS/MS）快速检测动物尿液中的莱克多巴胺和克伦特罗分析方法。样品经SCX柱净化浓缩后，以V（乙腈）+V（5 mmol/L乙酸铵）=65+35为流动相，采用Kromasil CN色谱柱分离，通过电喷雾（ESI）离子源电离多反应监测(MRM)对莱克多巴胺和克伦特罗作定性和定量分析。在1.0至500.0 μg/L的范围内线性良好(r>0.999)。在0.5、4.0、40.0、80.0、200.0 μg/L添加水平下，回收率在89.7～103.3%,相对标准偏差为2.0～7.8%。检测限（S/N=5）为0.1 μg/L。

Abstract:

The HPLC-MS/MS method was presented to analyze ractopamine and clenbuterol in Animal urine. The sample was purified and concentrated by SCX column, chromatographic conditions included a Kromasil CN column with the mobile phase of V(acetone)+V(5mmol/Lammonium acetate)=65+35. Qualitative and quantitative analysis of the ractopamine and clenbuterol which ionized by electrospray ionization interface(ESI) was carry on through MRM mode. There was a good linear correlation in the range of 1.0~500.0 μg/L(r>0.999).The recoveries ranged from 89.7% to 103.3% at the add concentration level 0.5、4.0、40.0、80.0、200.0 μg/L. The RSD was between 2.0% and 7.8%.Its limit of detection was 0.1 μg/L.