雏鸭接种Ⅰ型鸭肝炎鸡胚化弱毒MY后血清生化指标的动态变化

Dynamic Changes of Serum Biochemical Indexes in Ducklings Infected with Chicken Embryo attenuated strain MY Duck Hepatitis Virus Type I

关键词:  Ⅰ型鸭肝炎鸡胚化弱毒，血清生化指标，动态变化

Key words:  chicken embryo attenuated strain MY of duck hepatitis virus type I, serum biochemical indexes, dynamic changes

摘要:

用Ⅰ型鸭肝炎鸡胚化弱毒MY 人工接种4日龄雏鸭，于接种后6、12、24、48、72、96、144、168、192h及14d采集接种鸭的血液分离血清，进行生化指标测定，并与同期接种的弱毒疫苗HY、标准毒及对照组的血清生化指标进行比对。与对照组比较：MY组和HY组的白蛋白在接种后24h明显降低，48h差异极显著；血清谷丙转氨酶于24h，144h时段明显升高，且MY组持续至192h。标毒组则表现为接毒后，血清葡萄糖于24h~96h降低, 甘油三酯于72h、192h显著升高 ；血清总蛋白72h、 96h、144h，白蛋白24h~144h，球蛋白72h~ 96h，144h明显降低；谷丙转氨酶于24h~ 192h，血清谷草转氨酶在24-72h显著升高；尿酸72h呈一过性升高。表明：感染Ⅰ型鸭肝炎鸡胚化弱毒MY与弱毒疫苗HY的血清生化变化规律相似。

Abstract:

4 days old ducklings were infected with chicken embryo attenuated strain MY of duck hepatitis virus type I. Serum biochemical indexes of MY group, attenuated strain HY group, standard virus group and control group were measured at 6h, 12h, 24h, 48h, 72h, 96h, 114h, 168h, 192h and 14d after the infection. Compared to the control group, the content of ALB in MY group and HY group decreased distinctly at 24h after infection, and it was significantly lower at 48h after infection. The activities of ALT in MY group and HY group increased evidently at 24h and 144h after infection, and the activities of ALT in MY group maintaind to 192h. In standard virus group, the concentration of GLU dropped from 24h to 96h after infection, while the concentration of TG rose significantly. The content of TP at 72h, 96h and 144h after infection, the content of ALB at 24h and 144h after infection and the content of GLO from 72h to 96h and at 144h after infection reduced obviously. The activities of ALT from 24h to 192h and AST from 24h to 72h enhanced significantly. The concentration of uric acid augmented momently at 72h after infection. The results suggested that the change orderliness of serum biochemical indexes in ducklings infected with chicken embryo attenuated strain MY of duck hepatitis virus type I was similar to the ducklings’ infected with attenuated strain HY.