猪瘟E2亚单位联苗的免疫持续期及效力研究

Study on the Duration and Efficacy of Combined Immunization with Classical Swine Fever E2 Subunit Vaccine

关键词:  猪瘟亚单位联苗  免疫持续期  效力

Key words:  combined subunit vaccine against classical swine fever  immune duration  efficacy

摘要:

将猪瘟、猪伪狂犬病基因工程二联灭活疫苗(E2+gB+gD，CHO细胞源)免疫断奶仔猪，于一免后第7日、14日、21日，二免后第7日、14日、21日、42日、2个月、3个月、4个月、5个月、6个月、7个月、9个月、11个月、13个月采血，进行猪瘟ELISA抗体效价检测，并在二免后第13个月进行猪瘟攻毒保护试验。结果显示：免疫组免疫后第14日至2个月，猪瘟中和抗体水平处于上升期，在免疫第13个月抗体水平仍维持在较高水平，阻断率80%以上；二免后第13个月的疫苗免疫组猪瘟攻毒可达到5/5头保护，保护率100%，未免疫攻毒对照组5/5头发病且死亡。研究表明，猪瘟、猪伪狂犬病基因工程二联灭活疫苗(E2+gB+gD，CHO细胞源)猪瘟效力免疫持续期至少可以达到13个月，能够至少抵抗13个月猪瘟病毒的攻击。研究结果可为制定猪瘟E2亚单位联苗免疫程序提供依据。

Abstract:

In this study, the classical swine fever and porcine pseudorabies bivalent inactivated gene-engineered vaccine (E2+gB+gD, CHO cell-derived) was used to immunize weaned piglets. Blood samples were collected on 7,14,21 days after the first immunization, and on 7,14,21,42 days, 2 months, 3 months, 4 months, 5 months, 6 months, 7 months, 9 months, 11 months, and 13 months after the second immunization to conduct the detection of the antibody titer of classical swine fever by ELISA. And the challenge protection test for classical swine fever was carried out in the 13th month after the second immunization. The results showed that From the 14th day to 2 months after immunization in the immunized group, the level of neutralizing antibodies against classical swine fever was in a rising stage, and the antibody level still remained at a relatively high level in the 13th month after immunization. In the 13th month after the second immunization, the vaccine-immunized group could achieve a protection of 5 out of 5 pigs against the challenge of classical swine fever, with a protection rate of 100%, while in the unimmunized challenged control group, all 5 out of 5 pigs got sick and died. The research indicates that the immunization duration of the classical swine fever potency of the classical swine fever and porcine pseudorabies bivalent inactivated gene-engineered vaccine (E2+gB+gD, CHO cell-derived) can reach at least 13 months, and it can resist the attack of classical swine fever virus for at least 13 months. The time of resisting the attack of classical swine fever virus can provide a basis for formulating the immunization program.