鹅源坦布苏病毒的分离鉴定及分子遗传进化分析

Isolation, Identification and Genetic Evolution Analysis of Tembusu Virus From Goose

关键词:  鹅  坦布苏病毒  培养特性  E基因  遗传进化分析

Key words:  goose  Tembusu virus  cultural character  E gene  genetic evolution analysis

摘要:

2023年山东省菏泽市一鹅场雏鹅出现软脚、瘫痪、精神麻痹的症状，为确定该鹅场的发病原因，对送检的病死雏鹅进行剖检和实验室诊断，通过采集发病鹅肝脏、脾脏、脑等组织进行病原分离，通过病理剖检变化、RT-PCR检测、病毒的分离、基因遗传进化分析等方法进行鉴定。结果显示，从发病鹅场分离到一株鹅源坦布苏病毒(Tembusu virus，TMUV)，命名为SDHZ-G株，SDHZ-G株毒力较强，可致死鸭胚，ELD50为10-3.6/0.2mL。分离株与参考毒株E基因的核苷酸同源性在89.8%~99.7%之间，氨基酸同源性均在95.6%~99.8%之间，未发生明显遗传变异。该分离株属于TMUV基因2型2.2分支，是山东地区主要流行分支。本研究明确了引起该鹅场发病的病原为TMUV，并分析了致病原TMUV的遗传进化特点，为该鹅场雏鹅疫病防治提供了参考。

Abstract:

In 2023, goslings from a goose farm in Heze city Shandong province experienced symptoms of soft feet, paralysis, and mental paralysis. To determine the cause of the disease in the goose farm, this study conducted autopsy and laboratory diagnosis on the dead goslings sent for examination. This study isolated pathogens from the liver, spleen, brain, and other tissues of infected geese in the farm, and identified them through pathological dissection, RT-PCR detection, virus isolation and genetic evolution analysis. The results showed that a Tembusu virus (TMUV, SDHZ-G strain) was isolated from the goose farm. The SDHZ-G strain was highly virulent and lethal to duck embryos, and ELD50 was 10-3.6/0.2mL. The nucleotide similarity of the E gene was from 89.9% to 99.7%, and the amino acid similarity was from 95.6% to 99.8%, without significant genetic variation. This isolate belongs to the genotype 2.2 clade and is the main endemic clade in Shandong. This study identified the TMUV was the pathogen that causing the disease in the goose farm, and analyzed the genetic evolution characteristics of the pathogen TMUV, providing a reference for the prevention and treatment of gosling diseases in this farm.