猪瘟病毒单克隆抗体的研究及应用进展

Advances in the research and application of monoclonal antibodies against Classical Swine Fever

关键词:  猪瘟病毒  单克隆抗体  抗原表位  诊断

Key words:  swine fever virus  monoclonal antibodies  antigenic epitopes  diagnosis

摘要:

单克隆抗体是由单一B细胞克隆产生的高度均一、仅针对某一特定抗原表位的抗体，在疫病鉴别诊断和预防中发挥着重要作用。1986年Wensvoort等首次获得CSFV的单克隆抗体，此后，国内外制备了许多针对CSFV的单抗，用于免疫学研究及临床检测应用。本文对国内外制备的抗CSFV E2、Erns和NS3蛋白的单克隆抗体及其在CSFV抗原性分析、抗原表位研究及猪瘟诊断和鉴别中的应用等方面的最新进展进行了综述，以期为猪瘟病毒相关研究提供依据。

Abstract:

Monoclonal antibodies are highly homogeneous antibodies produced by a single B-cell clone against a specific antigenic epitope, and play an important role in differential diagnosis and prevention of epidemics.In 1986, Wensvoort et al. obtained the first monoclonal antibody against CSFV, and since then, a number of monoclonal antibodies against CSFV have been prepared both at home and abroad and have been used in immunology research and clinical testing applications. In this paper, the monoclonal antibodies against CSFV E2, Erns and NS3 proteins prepared at home and abroad and their recent progress in the analysis of CSFV antigenicity, antigenic epitope research, and application in the diagnosis and differentiation of swine fever are reviewed, with a view to providing a basis for the research related to swine fever virus.