高效液相色谱法同时测定复方磺胺二甲嘧啶钠可溶性粉中磺胺二甲嘧啶钠和甲氧苄啶的含量

Simultaneous determination of sulfamethazine sodium and trimethoprim in compound sulfadiazine sodium powder by HPLC

关键词:  高效液相色谱法  磺胺二甲嘧啶钠  甲氧苄啶

Key words:  HPLC  sulfamethazine sodium  trimethoprim

摘要:

建立高效液相色谱法同时测定复方磺胺二甲嘧啶钠可溶性粉中磺胺二甲嘧啶钠和甲氧苄啶的方法。采用十八烷基硅烷键合硅胶为填充剂，以水-乙腈-三乙胺（799：200：1）（用冰醋酸调节pH值至5.9）为流动相，流速1.0 mL/min，柱温30℃，检测波长为240 nm。结果显示，磺胺二甲嘧啶钠和甲氧苄啶2种成分线性关系良好（r>0.999），平均加样回收率分别为99.81%和99.25%，精密度、稳定性和耐用性均小于2.0%，符合方法学要求。该方法简便、稳定、准确，可用于复方磺胺二甲嘧啶钠可溶性粉的质量控制。

Abstract:

To establish an HPLC method for the Simultaneous determination of sulfamethazine sodium and trimethoprim in compound sulfadiazine sodium powder. The separation was developed on C18 column with water-acetonitrile-triethylamine (799:200:1) (pH5.9) as mobile phase. The column temperature was 30℃, the flow rate was 1.0mL/min, and the detection wavelength at 240 nm. The results showed that there were good linear relationships for sulfadiazine sodium and trimethoprim (respectively was r>0.999), the average recovery rate were 99.81% and 99.25%. The RSD of precision, stability and durability was all less than 2.0%, which met the requirements of method validation. This method is simple, stable, accurate, which could be used to the quality of compound sulfadiazine sodium powder.