| 摘要: |
| 为研究禽多杀性巴氏杆菌类兽用生物制品效力评价用标准菌株,制备了一批禽多杀性巴氏杆菌CVCC44801株,并对菌种的形态及生化特性、培养特性、血清学特性、真空度、纯粹、剩余水分、毒力等进行检定,进一步评估了冻干菌菌数的稳定性,对于冻干菌与新鲜培养菌液的毒力进行了比较。。试验结果表明,该冻干菌种的形态及生化特性、培养特性、血清学特性、真空度、纯粹、剩余水分、毒力均符合《中华人民共和国兽用生物制品规程》二〇〇〇版质量标准的规定。冻干菌菌数保持稳定,并且与新鲜培养的攻毒菌液相比,毒力并无差异。本研究为禽多杀性巴氏杆菌类兽用生物制品效力评价用标准菌株的制备和检定提供参考依据。 |
| 关键词: 禽多杀性巴氏杆菌CVCC44801株 毒力 效力评价 |
| DOI： |
| 投稿时间：2023-09-08修订日期：2023-12-13 |
| 基金项目:兽药行业公益性重点专项(GY202104) |
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| Study on standardization of Pasteurella multocida for the efficacy evaluation of veterinary biological productsFENG Yan,ZHANG Yi-zhi,WANG Xiu-li,WANG Jia,LIU Yan, |
|
| (China Institute of Veterinary Drug Control) |
| Abstract: |
| In order to study the standard strains of Pasteurella multocida for the efficacy evaluation of veterinary biological products, a batch of avian Pasteurella multocida CVCC44801 strains were prepared, and the morphology, biochemical characteristics, culture characteristics, serological characteristics, vacuum degree, purity, residual water and virulence of the strains were tested, and the stability of the freezer-dried bacteria was further evaluated. The e virulence of freeze-dried bacteria was compared with that of freshly cultured bacteria. The results showed that the morphology, biochemical characteristics, culture characteristics, serological characteristics, vacuum degree, purity, residual water and virulence of the lyophilized strain were in accordance with the quality standard of the Code of the People"s Republic of China for Veterinary Biological Products 2000 edition. The number of freeze-dried bacteria remained stable, and the |
| Key words: avian Pasteurella multocida CVCC44801 strain Virulence Effectiveness evaluation |
