卡洛芬注射液对靶动物奶牛安全性试验研究

Target Animal Safety Study on Carprofen Injection in Cows

关键词:  卡洛芬注射液  奶牛  安全性

Key words:  carprofen injection  cow  safety

摘要:

为研究卡洛芬注射液对靶动物奶牛的安全性，将20头健康奶牛随机分为4组，每组5头，各组试验奶牛分别按0、1倍推荐剂量（1.4mg/kg B.W.）、3倍推荐剂量（4.2mg/kg B.W.）、5倍推荐剂量（7.0mg/kg B.W.）单剂量皮下注射卡洛芬注射液，连续给药3d。试验期间观察记录各组奶牛的饮食、精神、粪便情况及试验前后的体重变化，测定给药前、给药后第1d及给药后第3d各组奶牛的血液生理生化指标，同时在给药后第3d进行剖检，测定脏器指数，观察病理及组织学变化。结果显示，卡洛芬注射液各剂量组奶牛的临床体征、血液生理生化指标、剖检及组织显微结构等均未见明显异常，未出现中毒症状及死亡。与对照组相比，3倍剂量组和5倍剂量组血液生理指标中血小板平均宽度差异显著（P＜0.05），5倍剂量组血液生化指标白蛋白数量明显增加（P＜0.05），但所有指标均在正常范围内波动。结果表明，卡洛芬注射液在临床上推荐以1.4mg/kg B.W.给药对靶动物奶牛是安全的。

Abstract:

In order to evaluate the target animal safety on carprofen injection in cow, 20 healthy cows were divided into four groups randomly, each group was given carprofen injection at a single dose of 0, recommended dosage（1.4mg/kg B.W.）, 3 times of recommended dosage（4.2mg/kg B.W.）and 5 times of recommended dosage（7.0mg/kg B.W.）respectively. Mental state, feed intake, water drinking and fecal examination were observed and recorded daily throughout the experimental period, and the body weight changes during the experiment were recorded. Before administration and twenty-four hours after the last administration, blood and serum sample were collected for hematological and biochemical assays. And selected organs were obtained for organ coefficients and histopathological analysis. The results showed that the clinical symptoms, blood biochemical and physiological indexes, dissection and tissue microstructure of different dose groups of carprofen injection were found no significant abnormalities and no toxic symptoms or death were found. Compared with the control group，there was a significant difference in the average platelet width in blood physiological indicators between the 3 and 5 times of recommended dosage (P<0.05). Significant increase in the number of blood biochemical indicators albumin in the 5 times of recommended dosage (P<0.05). However, all indicators fluctuate within the normal range. In conclusion，the recommended dose(1.4mg/kg B.W) of carprofen injection was safe to cow．