红霉素高产菌株选育及其发酵条件的优化

Breeding of High Erythromycin-producing Strains and Optimization of Fermentation Conditions

关键词:  红霉素  高产菌株  诱变  选育  发酵条件  优化

Key words:  erythromycin  high yield strain  mutagenesis  fermentation processes  optimization

摘要:

为选育出红霉素(Erythromycin)发酵生产的优良菌株并建立其高效发酵条件,以产红霉素的红色糖多孢菌(Saccharopolyspora erythraea)SE-2207菌株作为原始菌株,利用EMS(甲基磺酸乙酯)、UV(紫外诱变)和ARTP(常压室温等离子体诱变)诱变的方法开展优良菌种选育,比较了三种方法的诱变效果,选育出1株遗传稳定、且对红霉素碱耐受性强的菌株SEM-7,其红霉素发酵效价达到8368 mg/L,比原始菌株提高了25.5%。而且,SEM-7菌株细胞酰基辅酶A合成酶活力显著高于原始菌株。通过对发酵培养基中添加正丙醇、豆油的浓度进行优化筛选,使SEM-7菌株500 L中试发酵效价达到9155 mg/L。研究结果对提升红霉素工业化发酵生产水平具有重要意义。

Abstract:

In order to select a good strain and optimize fermentation processes for erythromycin fermentation, Saccharopolyspora erythraea SE-2207 strain producing erythromycin was used as the original strain. SE-2207 strain was mutagenized by mutagenesis EMS (ethyl methanesulfonate), UV (ultraviolet) and ARTP (atmospheric pressure room temperature plasmawas), respectively. And the mutagenic effects of the three methods were compared. After mutagenesis, SEY-7 strain with good genetic stability and high erythromycin base tolerance was screened out. Its mycophenolic acid fermentation potency was up to 8368 mg/L, which was 25.5% higher than that of the original strain. Based on screening concentration of N-propanol and soybean oil in the medium, the fermentation processes were optimized and made the fermentation titer of 500 L up to 9155 mg/L. The results were of great significance to improve fermentation production level of erythromycin.