HPLC和原子吸收光谱法测定黄芩苷锌含量的比较研究

A Comparative Study of the Determination of Zinc Baicalin Content by HPLC and Atomic Absorption Spectrometry

关键词:  黄芩苷锌  含量测定  高效液相色谱法  原子光谱法

Key words:  baicalin-zinc complex  determination  HPLC  atomic absorption spectrophotometry

摘要:

为了比较黄芩苷锌含量测定的不同方法，建立更加简单、快速的含量测定方法，分别采用高效液相色谱法法和原子吸收光谱法对黄芩苷锌原料药的含量测定进行研究。结果表明，HPLC测定黄芩苷锌线性范围为10.0~200.0 μg/mL，相关系数r=0.9999，精密度(RSD=0.08%)良好；原子吸收光谱法线性范围为0~2.0 μg/mL，相关系数r=0.9983，精密度(RSD=0.13%)良好，但前处理过程过于复杂，耗时太长。因此更推荐HPLC法。

Abstract:

In order to compare the different methods for the determination of baicalin-zinc complex and establish a simpler and faster content determination method, HPLC method and atomic absorption spectrophotometry method were used to study the content determination of baicalin-zinc complex. The linear range of baicalin-zinc complex was 10.0~200.0 μg/mL (r=0.9999), and the precision (RSD=0.08%) was good by HPLC. The linear range of atomic absorption spectrometry was 0~2.0 μg/mL (r=0.9983), and the precision (RSD=013%) was good, but the pretreatment process was too complex and time-consuming. The HPLC method was simpler, faster and accurater, which could be used to determine the content of baicalin-zinc complex.