纳米金-核酸适配体可视化检测蜂蜜中恩诺沙星的方法研究

Determination of visual detection of enrofloxacin in animal products by nano gold nanoparticles nucleic acid aptamer

关键词:  恩诺沙星  纳米金  核酸适配体  快速检测

Key words:  Enrofloxacin  Gold nanoparticles  Nucleic acid aptamer  Rapid detection

摘要:

目的 建立纳米金-核酸适配体可视化检测畜产品中恩诺沙星的方法。方法 首先利用还原法合成纳米金（gold nanoparticles, AuNPs）颗粒，利用紫外分光光度计对实验原理进行验证，优化了实验条件，建立了恩诺沙星浓度和特定波长下吸光度值之间的关系式。同时也验证了该方法的特异性。结果 所得线性回归方程在低浓度时为y=0.6377x+0.89495，R2=0.99026，方法检出限为0.018 CFU/mL；所得线性回归方程在高浓度时为y=0.04288x+0.95578，R2=0.99812，方法检出限为0.265 CFU/mL。方法除可用来检测恩诺沙星外，还可用于检测盐酸恩诺沙星，具有一定的特异性。对恩诺沙星实际样品测定的回收率为95.5%~97.8%，相对标准偏差小于10%。结论 方法操作方便简便，成本低，检测时间短，可用于畜产品中恩诺沙星快速定量检测。

Abstract:

Objective To establish a method for the visual detection of enrofloxacin in animal products by nano-gold-nucleic acid aptamer. Methods First, the reduction method was used to synthesize gold nanoparticles (AuNPs), and the experimental principle was verified by an ultraviolet spectrophotometer, the experimental conditions were optimized, and the relationship between the concentration of enrofloxacin and the absorbance value at a specific wavelength was established. At the same time, the specificity of the method was verified. Results The linear regression equation obtained at low concentration was y=0.6377x+0.89495, R2=0.99026, and the detection limit of the method was 0.018 CFU/mL; at high concentration, the linear regression equation obtained was y=0.04288x+0.95578, R2=0.99812, The detection limit of the method is 0.265 CFU/mL. In addition to detecting enrofloxacin, the method can also be used to detect enrofloxacin hydrochloride, which has certain specificity. The recovery of actual samples of enrofloxacin was 95.5%-97.8%, and the relative standard deviation s (RSD) was less than 10%. Conclusion The method was convenient and simple to operate, low cost, short detection time, and can be used for rapid quantitative detection of enrofloxacin in animal products.