22味中药及其复方对奶牛乳房炎病原菌金黄色葡萄球菌的体外抑菌试验研究

In vitro antibacterial test of 22 traditional Chinese medicines and it’s compound preparations on pathogenic Staphylococcus aureus of dairy cow mastitis

关键词:  中药  奶牛乳房炎  金黄色葡萄球菌  体外抑菌

Key words:  traditional Chinese medicine  cow mastitis  Staph. aureus  in vitro antibacterial

摘要:

为了筛选有效中药抑制奶牛乳房炎病原菌金黄色葡萄球菌，采用药敏片法测定黄芩等22味中药的体外抑菌效果。采用水提法制备中药药液，浓缩为生药1 g?mL-1，测定22味中药对金黄色葡萄球菌的体外抑菌圈直径、最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。结果表明：黄芩和地榆对金黄色葡萄球菌呈高度敏感，白术、丹皮等5味中药均属中度敏感。选取体外抑菌效果最好的黄芩和地榆加上白术、丹皮组成不同比例复方进行抑菌实验，结果表明：复方Ⅱ和复方Ⅶ的抑菌和杀菌效果最佳，其MIC和MBC均为31.25 mg?mL-1。

Abstract:

In order to screen out effective traditional Chinese medicine to inhibit Staphylococcus aureus (Staph. aureus) of dairy cow mastitis’ s pathogenic bacteria, the antibacterial effect of 22 traditional Chinese medicine on Staph. aureus in vitro was determined by drug sensitive tablet method. The liquid of traditional Chinese medicine was prepared by water extraction to concentrate into crude medicine of 1 g?mL-1. The inhibitory circle diameter in vitro, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 22 traditional Chinese medicine against Staph. aureus were measured. The results showed that Scutellaria baicalensis and Sanguisorba officinalis were highly sensitive to Staph. aureus. Five herbs, including Atractylodes macrocephala Koidz and Tree Peony Bark were moderately sensitive. The antibacterial experiments were carried out with different proportions of Scutellaria baicalensis baicalensis and sanguisorba with Atractylodes macrocephala Koidz macrocephala and cortex paeoniae. The results showed that compound II and compound VII had the best antibacterial and bactericidal effects, and their MIC and MBC were both 31.25mg?mL-1.