湖南省奶牛源金黄色葡萄球菌分离鉴定与耐药性分析

Isolation, identification and drug resistance analysis of Staphylococcus aureus from dairy cows in Hunan Province

关键词:  奶牛源  金黄色葡萄球菌  细菌鉴定  耐药性：耐甲氧西林金黄色葡萄球菌

Key words:  dairy cow source  Staphylococcus aureus  Identification of bacteria  MRSA

摘要:

为了掌握湖南省内奶牛源金黄色葡萄球菌的耐药情况，指导临床合理用药,抽取牛乳样品，通过对细菌的分离培养和纯化，采用基质辅助激光电离飞行时间质谱仪和全自动生化鉴定仪进行鉴定，并对分离菌株进行18种抗菌药物耐药性检测;结合耐药表型和mecA基因检测判定MRSA菌株。结果显示，2020-2021年从湖南地区257份牛乳样品中分离出金黄色葡萄球菌39株，89.74%的临床分离菌株表现为不同程度耐药，其中对青霉素耐药最为严重,耐药率达82.05%；对红霉素、克林霉素、替米考星、庆大霉素、头孢西丁耐药率在20%-35%之间；对多西环素及万古霉素绝对敏感,耐药率为0。38.46%分离菌株表现为多重耐药，以7重耐药最多。鉴定出19株MRSA，其中包含15株OS-MRSA和4株OR-MRSA。本研究可为湖南省内奶牛企业制定合理用药措施提供依据。

Abstract:

In order to master the drug resistance of Staphylococcus aureus from dairy cows in Hunan Province and guide clinical rational drug use, raw milk samples were taken, the bacteria were isolated, cultured and purified, identified by microbial mass spectrometry and automatic biochemical identification instrument, and 18 kinds of antimicrobial drug resistance of isolated strains were detected. MRSA strains were identified by drug resistance phenotype and mecA gene detection. The results showed that 39 strains of Staphylococcus aureus were isolated from 257 milk samples in Hunan province during 2020-2021. 89.74% of the clinical isolates showed different degrees of drug resistance, among which penicillin resistance was the most serious, with a drug resistance rate of 82.05%. The drug resistance rate to erythromycin, clindamycin, temicorin, gentamicin and cefoxitin ranged from 20% to 35%. Absolutely sensitive to doxycycline and vancomycin which drug resistance rate was 0. 38.46% isolates showed multiple drug resistance, with 7 strains being the most resistant. Nineteen strains of MRSA were identified, including 15 OS-MRSA and 4 OR-MRSA.This study can provide basis for dairy enterprises in Hunan province to formulate rational drug use measures.