多拉菌素的复合诱变及发酵条件优化

Compound Mutagenesis of Doramectin and Optimization of Fermentation Conditions

关键词:  多拉菌素  复合诱变

Key words:  doramectin  compound mutagenesis

摘要:

为通过诱变获得遗传性状稳定的多拉菌素高产菌株，对多拉菌素产生菌RDL19-1进行多种诱变处理包括常压室温等离子(ARTP)诱变，紫外(UV)诱变，及ARTP-UV复合诱变。该菌株接种瓶后于28 ℃摇床培养32 h，以10%的接种量转入装量为35 mL/300 mL 发酵瓶中，在28 ℃条件下发酵培养14 d，多拉菌素发酵产量达到了1390 μg/mL，相较于诱变前提高了4倍。通过对多拉菌素产生菌RDL19-1进行ARTP-UV复合诱变，获得了一株遗传性状稳定的高产菌株。

Abstract:

To obtain a high-yield doramectin strain with stable genetic traits by mutagenesis, the doramectin-producing strain RDL19-1 was subjected to various mutagenesis treatments such as atmospheric room temperature plasma (ARTP) mutagenesis, ultraviolet (UV) mutagenesis, and ARTP-UV combined mutagenesis. After inoculating the flask, the strain was cultured on a shaker at 28 ℃ for 32 hours, and transferred to a 35 mL/300 mL fermentation flask with 10% of the inoculum, and fermented and cultured at 28 ℃ for 14 days. The fermentation yield of doramectin reached 1390 μg/mL, which is 4 times higher than that before mutagenesis. By ARTP-UV compound mutagenesis of doramectin-producing strain RDL19-1, a high-yielding strain with stable genetic characteristics was obtained.