HPLC-PDA法测定兽用中药液体制剂中非法添加的四种解热镇痛类药物

Determination of four antipyretic and analgesic drugs illegally added in liquid preparations of veterinary traditional Chinese medicine by HPLC-PDA

关键词:  解热镇痛药  中药液体制剂  非法添加  HPCL-PDA

Key words:  analgesic-antipyretic  liquid preparation of chinese medicine  Illegally added  HPCL-PDA

摘要:

实验建立了兽用中药液体制剂中非法添加对乙酰氨基酚、安乃近、氨基比林、安替比林四种解热镇痛类药物的高效液相色谱-二极管阵列检测方法。方法采用CAPCELL PAK MGⅡ S5 C18 (4.6 mm × 150 mm,5 μm)色谱柱,柱温30℃,流动相为磷酸盐缓冲液和乙腈(84:16),流速1.0ml/min,进样体积10 μl,采集波长范围为190 nm~400 nm,检测波长229 nm。结果显示,对乙酰氨基酚、安乃近、安替比林、氨基比林在浓度分别在1.17~117.28、2.03~202.92、5.58~111.60、2.42~120.80 μg/ml 范围内线性良好,相关系数R_²分别为0.9986、0.9999、0.9980、0.9993,平均加标回收率(n=5)分别为93.15%~104.25%、93.79%~104.62%、96.96%~99.17%、94.66%~99.83%,RSD分别为0.46%~2.29%、0.30%~3.15%、0.12%~0.94%、0.37%~1.42%(n=5)。该方法便捷,快速,准确,可用于兽用中药液体制剂中非法添加四类解热镇痛类药物的筛查检测。

Abstract:

This experiment established a HPLC-PDA detection method for the illegal addition of four antipyretic and analgesic drugs: paracetamol, paraben, aminopyrine, and antipyrine in liquid preparations of veterinary traditional Chinese medicine. The method used a CAPCELL PAK MGⅡ S5 C18 (4.6 mm × 150 mm, 5 μm) chromatographic column, the column temperature was 30℃, the mobile phase was phosphate buffer and acetonitrile (84:16), the flow rate was 1.0ml/min, and the injection volume 10 μl, the collection wavelength range is 190 nm~400 nm, and the detection wavelength is 229 nm.. The results showed that acetaminophen, analgin, antipyrine, and aminopyrine had good linearity in the concentration range of 1.17~117.28, 2.03~202.92, 5.58~111.60, 2.42~120.8 μg/ml, respectively. The correlation coefficients R_² were 0.9986, 0.9999, 0.998, 0.9993, and the average recovery rates (n=5) were 93.15%~104.25%, 93.79%~104.62%, 96.96%~99.17%, 94.66%~99.83%, RSD They were 0.46%~2.29%, 0.30%~3.15%, 0.12%~0.94%, 0.37%~1.42% (n=5). The method is convenient, fast, and accurate, and can be used for screening and testing four types of antipyretic and analgesic drugs illegally added to liquid preparations of veterinary traditional Chinese medicine.