动物源食品中氨曲南残留检测方法的建立

Establishment of a Detection Method for Aztreonam Residues in Foods of Animal Origin

关键词:  动物源食品  氨曲南  高效液相色谱-串联质谱

Key words:  Animal original food  Aztreonam  HPLC-MS/MS

摘要:

建立了高效液相色谱-串联质谱法（HPLC-MS/MS）测定动物源食品中氨曲南残留的方法。样品经磷酸缓冲盐溶液提取后，过固相萃取柱净化，进HPLC-MS/MS检测。结果表明，该方法的标准工作曲线线性良好（R2>0.995），样品加标回收率均在70 %-110 %之间，相对标准偏差(RSD)小于10 %，方法的检出限为1.0 μg/kg，定量限为2.0μg/kg。该方法前处理简便、可靠、灵敏度高，可适用于动物源食品中氨曲南残留的测定。

Abstract:

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of aztreonam residues in animal-origin foods was established in this study. The sample was firstly extracted with phosphate buffered saline solution, and purified by solid phase extraction column, and then the extracted solution was detected by HPLC-MS/MS. The results showed the the linearity of the standard work curve was good (R2>0.995), and the average sample recovery rates were 70%~110%, and the relative standard deviation of the method was less than 10%. The limition of detection and quantitation was 1.0μg/kg and 2.0 μg/kg, recpectively. The method was simple, reliable, and sensitive, and can be applied to the determination of aztreonam residues in foods of animal origin.