HPLC同时测定翻黄合剂中芍药苷和盐酸小檗碱含量

Content Determination of Paeoniflorin and Berberine Hydrochloride in Fanhuang Mixture by HPLC

关键词:  翻黄合剂  芍药苷  盐酸小檗碱  HPLC

Key words:  Fanhuang mixture  paeoniflorin  berberine hydrochloride  HPLC

摘要:

目的 建立同时测定翻黄合剂中芍药苷和盐酸小檗碱含量的HPLC方法。方法 采用中性氧化铝柱对供试品溶液进行处理,使目标峰不被杂质峰干扰。采用YMC-Pack Pro C18色谱柱(250×4.6mm),流动相为乙腈-0.15%磷酸溶液(梯度洗脱),流速为1.0mL.min^-1,柱温为26℃,检测波长为245nm。结果 芍药苷和盐酸小檗碱的进样量分别在0.307～4.920μg(r=0.9996)和0.064～1.025μg(r=0.9998)范围内与峰面积呈良好的线性关系,平均回收率分别为91.10%(RSD=1.75%,n=6)和92.06%(RSD=1.69%,n=6)。结论 该法简单、可靠,可以同时检测翻黄合剂中芍药苷和盐酸小檗碱的含量,为翻黄合剂的质量控制提供了依据。

Abstract:

Objective To establish an HPLC method for the content determination of paeoniflorin and berberine hydrochloride in Fanhuang mixture. Methods Neutral alumina column was used to treat the sample solution so that the target peak was not disturbed by the impurity peak. Used YMC-Pack Pro C18 analysis column(250×4.6 mm),mobile phase of acetonitrile-0.15% phosphoric acid aqueous solution(gradient elution),the flow rate was 1.0 mL.min^-1,the column temperature was 26℃,the detection wavelength was 245 nm. Results The linear ranges of paeoniflorin and berberine hydrochloride were 0.307～4.920 μg(r=0.9996) and 0.064～1.025 μg(r=0.9998) respectively,and their average recovery rates were 91.10%(RSD=1.75%,n=6) and 92.06%(RSD=1.69%,n=6)respectively. Conclusion The method is simple and reliable,and can simultaneously detect the content of paeoniflorin and berberine hydrochloride in Fanhuang mixture,providing a basis for the quality control of Fanhuang mixture.